Puss in Boots discovers that his passion for adventure has taken its toll: He has burned through eight of his nine lives, leaving him with only one life left. Puss sets out on an epic journey to find the mythical Last Wish and restore his nine lives.

Thunder, an abandoned young cat seeking shelter from a storm, stumbles into the strangest house imaginable, owned by an old magician and inhabited by a dazzling array of automatons and gizmos. Not everyone welcomes the new addition to the troupe as Jack Rabbit and Maggie Mouse plot to evict Thunder. The situation gets worse when the magician lands in hospital and his scheming nephew sees his chance to cash in by selling the mansion. Our young hero is determined to earn his place and so he enlists the help of some wacky magician's assistants to protect his magical new home.